› 6iok

Electron Microscopy
3.64Å resolution

Cryo-EM structure of multidrug efflux pump MexAB-OprM (0 degree state)

Released:
DOI: 10.2210/pdb6iok/pdb
PDB entry 6iok coloured by chain, front view.
PDB entry 6iok coloured by chain, side view.
PDB entry 6iok coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structures of the wild-type MexAB-OprM tripartite pump reveal its complex formation and drug efflux mechanism.
Tsutsumi K, Yonehara R, Ishizaka-Ikeda E, Miyazaki N, Maeda S, Iwasaki K, Nakagawa A, Yamashita E
Nat Commun 10 1520 (2019)
PMID: 30944318
Related structures: EMD-9695

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-156448 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Multidrug resistance protein MexA Chains: I, J, K, L, M, N
Molecule details ›
Chains: I, J, K, L, M, N
Length: 362 amino acids
Theoretical weight: 38.79 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P52477 (Residues: 25-383; Coverage: 100%)
Gene names: PA0425, mexA
Sequence domains:
Outer membrane protein OprM Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 474 amino acids
Theoretical weight: 51.74 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51487 (Residues: 18-485; Coverage: 100%)
Gene names: PA0427, oprK, oprM
Sequence domains: Outer membrane efflux protein
Structure domains:
Multidrug resistance protein MexB Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 1054 amino acids
Theoretical weight: 113.95 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P52002 (Residues: 1-1046; Coverage: 100%)
Gene names: PA0426, mexB
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.64Å
Relevant EMDB volumes: EMD-9695
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Structural and Functional Diversity of Resistance-Nodulation-Cell Division Transporters.
Klenotic et al. (2021)
13 more

6 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
5 more