X-ray diffraction
2Å resolution

The structure of the H109A mutant of UdgX in complex with uracil


Function and Biology Details

Reaction catalysed:
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Type-4 uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 22.42 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
  • Canonical: I7F541 (Residues: 7-215; Coverage: 97%)
Gene name: MSMEI_0259
Sequence domains: Uracil DNA glycosylase superfamily

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 39.151Å b: 51.158Å c: 40.957Å
α: 90° β: 92.19° γ: 90°
R R work R free
0.197 0.195 0.254
Expression system: Escherichia coli