X-ray diffraction
2.5Å resolution

Crystal Structure of human lysyl-tRNA synthetase L350H mutant

Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Lysine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 521 amino acids
Theoretical weight: 60.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q15046 (Residues: 70-581; Coverage: 86%)
Gene names: KARS, KARS1, KIAA0070
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P3221
Unit cell:
a: 152.18Å b: 152.18Å c: 106.32Å
α: 90° β: 90° γ: 120°
R R work R free
0.195 0.194 0.226
Expression system: Escherichia coli