6ihw

X-ray diffraction
1.55Å resolution

Crystal structure of bacterial serine phosphatase bearing R161K mutation

Released:
Source organism: Staphylococcus aureus

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PPM-type phosphatase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 30.11 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RL81 (Residues: 3-247; Coverage: 99%)
Gene names: BN1321_240063, BTN44_06615, C7P97_09885, CSC87_08725, DQV53_06510, E3A28_02540, E3K14_06010, EP54_08495, EQ90_08165, ERS072840_01404, FA040_04580, G0X12_02540, G0Z08_10970, G6Y10_11525, GF545_05680, GF559_04870, GO677_10370, GO746_09405, GO788_03515, GO793_10355, GO803_13910, GO821_05845, GO894_03380, GO915_11975, GO942_07250, HMPREF2819_02510, NCTC10654_01263, NCTC10702_01924, NCTC10988_01448, SAMEA1029528_00202, SAMEA1029547_01617, SAMEA1029553_01716, SAMEA103891215_01236, SAMEA103891286_00206, SAMEA103891321_00207, SAMEA103891529_00643, SAMEA103891571_01530, SAMEA1531680_01545, SAMEA1964876_01572, SAMEA1965205_01586, SAMEA1966505_01029, SAMEA1969349_01558, SAMEA1969845_01295, SAMEA1971706_01764, SAMEA1972827_01187, SAMEA2076212_01583, SAMEA2076218_01188, SAMEA2076220_01573, SAMEA2076226_00726, SAMEA2076463_01324, SAMEA2076464_01323, SAMEA2076470_01543, SAMEA2076472_01652, SAMEA2076478_01635, SAMEA2076480_01291, SAMEA2076481_01945, SAMEA2076743_01572, SAMEA2076745_01900, SAMEA2076746_01630, SAMEA2076747_01335, SAMEA2076749_01616, SAMEA2076751_01427, SAMEA2076752_01376, SAMEA2076755_01227, SAMEA2076756_01287, SAMEA2076758_01127, SAMEA2076759_01199, SAMEA2076761_01551, SAMEA2076762_01770, SAMEA2076763_01702, SAMEA2076764_01717, SAMEA2076765_01766, SAMEA2077023_01773, SAMEA2077025_01497, SAMEA2077027_01518, SAMEA2077029_01493, SAMEA2077031_01581, SAMEA2077034_01733, SAMEA2077035_01297, SAMEA2077039_01433, SAMEA2077040_01101, SAMEA2077041_01747, SAMEA2077044_01549, SAMEA2077045_01594, SAMEA2077046_01420, SAMEA2077293_01622, SAMEA2077294_01726, SAMEA2077295_01424, SAMEA2077297_01421, SAMEA2077300_01725, SAMEA2077301_01422, SAMEA2077302_01607, SAMEA2077303_01502, SAMEA2077307_01453, SAMEA2077832_01700, SAMEA2078252_01746, SAMEA2078256_01319, SAMEA2078307_01631, SAMEA2078308_01289, SAMEA2078553_01222, SAMEA2078558_01526, SAMEA2078560_01555, SAMEA2078569_01757, SAMEA2078570_01658, SAMEA2078572_01846, SAMEA2078824_01321, SAMEA2078837_01582, SAMEA2079048_01539, SAMEA2079051_01642, SAMEA2079277_01357, SAMEA2079291_01823, SAMEA2079503_01657, SAMEA2079507_01779, SAMEA2079512_01630, SAMEA2079517_01307, SAMEA2079724_01158, SAMEA2079727_00947, SAMEA2079728_01500, SAMEA2079732_01190, SAMEA2079946_01159, SAMEA2079949_01144, SAMEA2079951_01352, SAMEA2079952_01192, SAMEA2079957_01275, SAMEA2079958_01000, SAMEA2079960_01189, SAMEA2079961_00534, SAMEA2079968_01323, SAMEA2080329_01242, SAMEA2080330_01312, SAMEA2080334_01539, SAMEA2080433_01086, SAMEA2080812_01260, SAMEA2080898_02027, SAMEA2080900_01580, SAMEA2080904_01409, SAMEA2080913_01469, SAMEA2081043_01655, SAMEA2081053_01562, SAMEA2081054_01234, SAMEA2081055_01519, SAMEA2081060_00839, SAMEA2081211_01561, SAMEA2081213_01202, SAMEA2081218_01063, SAMEA2081341_01162, SAMEA2081342_00955, SAMEA2081349_01040, SAMEA2081359_01206, SAMEA2081362_01606, SAMEA2081468_01317, SAMEA2081474_01522, SAMEA2081475_01598, SAMEA2081476_00878, SAMEA2081479_01390, SAMEA2081480_01700, SAMEA2081560_01303, SAMEA2081561_01496, SAMEA2081564_01716, SAMEA2081567_01705, SAMEA2081568_01713, SAMEA2081569_01233, SAMEA2081570_01716, SAMEA2081571_01641, SAMEA2081572_01453, SAMEA2081573_01261, SAMEA2081575_01580, SAMEA2081577_01589, SAMEA2081578_01302, SAMEA2081579_01480, SAMEA2081581_01386, SAMEA2081582_01722, SAMEA2081673_01040, SAMEA2081674_01038, SAMEA4008573_01746, SAMEA4008676_01320, SAMEA70146418_01691, SAMEA958766_00788, SAMEA958770_01348, SAMEA958772_01188, SAMEA958778_00174, SAMEA958779_01267, SAMEA958785_00809, SAMEA958793_01014, SAMEA958798_01040, SAMEA958804_00817, SAMEA958810_01398, SAMEA958836_00263, SAMEA958838_00827, SAMEA958845_00939, SAMEA958846_01727, SAMEA958848_01751, SAMEA958855_00671, SAMEA958858_00408, SAMEA958898_00752, SAMEA958906_01165, SAMEA958924_01260, SAMEA958925_01281, SAMEA958951_01080, SAMEA958953_01066, SAMEA958961_01332, SAMEA958979_00765, SAMEA958987_01433, SAMEA958995_00755, SAST44_01247, prpC
Sequence domains: Protein phosphatase 2C

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 46.578Å b: 38.958Å c: 65.409Å
α: 90° β: 102.08° γ: 90°
R-values:
R R work R free
0.152 0.151 0.174
Expression system: Escherichia coli