6ift

X-ray diffraction
1.9Å resolution

KsgA from Bacillus subtilis in complex with SAM

Released:

Function and Biology Details

Reaction catalysed:
4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase A Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 32.76 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P37468 (Residues: 1-292; Coverage: 100%)
Gene names: BSU00420, ksgA, rsmA
Sequence domains: Ribosomal RNA adenine dimethylase

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 45.2Å b: 81.97Å c: 47.12Å
α: 90° β: 112.02° γ: 90°
R-values:
R R work R free
0.179 0.177 0.235
Expression system: Escherichia coli