6iek

X-ray diffraction
2.7Å resolution

Structure of RVFV Gn and human monoclonal antibody R12

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glycoprotein N Chains: A, D
Molecule details ›
Chains: A, D
Length: 316 amino acids
Theoretical weight: 34.94 KDa
Source organism: Rift Valley fever virus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P03518 (Residues: 154-469; Coverage: 27%)
Gene name: GP
Heavy chain of Fab R12 Chains: B, E
Molecule details ›
Chains: B, E
Length: 226 amino acids
Theoretical weight: 24.33 KDa
Source organism: Homo sapiens
Expression system: Mammalia
Structure domains: Immunoglobulins
Light chain of Fab R12 Chains: C, F
Molecule details ›
Chains: C, F
Length: 219 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Mammalia
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 97.756Å b: 108.07Å c: 107.418Å
α: 90° β: 90.34° γ: 90°
R-values:
R R work R free
0.227 0.225 0.258
Expression systems:
  • Insect cell expression vector pTIE1
  • Mammalia