› 6iea

X-ray diffraction
2Å resolution

Structure of RVFV Gn and human monoclonal antibody R13

Released:
DOI: 10.2210/pdb6iea/pdb
PDB entry 6iea coloured by chain, front view.
PDB entry 6iea coloured by chain, side view.
PDB entry 6iea coloured by chain, top view.
Source organisms:
Primary publication:
Neutralization mechanism of human monoclonal antibodies against Rift Valley fever virus.
Wang Q, Ma T, Wu Y, Chen Z, Zeng H, Tong Z, Gao F, Qi J, Zhao Z, Chai Y, Yang H, Wong G, Bi Y, Wu L, Shi R, Yang M, Song J, Jiang H, An Z, Wang J, Yilma TD, Shi Y, Liu WJ, Liang M, Qin C, Gao GF, Yan J
Nat Microbiol 4 1231-1241 (2019)
PMID: 30936489

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208963 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glycoprotein N Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.94 KDa
Source organism: Rift Valley fever virus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P03518 (Residues: 154-469; Coverage: 27%)
Gene name: GP
Sequence domains: Phlebovirus glycoprotein G1
R13 L chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
R13 H chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 23.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 42.21Å b: 97.107Å c: 112.504Å
α: 90° β: 96.3° γ: 90°
R-values:
R R work R free
0.183 0.181 0.225
Expression systems:
  • Insect cell expression vector pTIE1
  • Homo sapiens

Quick links

Citations

6 review citations

Rift Valley fever: biology and epidemiology.
Wright et al. (2019)
5 more