› 6ibg

X-ray diffraction
1.95Å resolution

Bacteriophage G20c portal protein crystal structure for construct with intact N-terminus

Released:
DOI: 10.2210/pdb6ibg/pdb
PDB entry 6ibg coloured by chain, front view.
PDB entry 6ibg coloured by chain, side view.
PDB entry 6ibg coloured by chain, top view.
Source organism: Thermus virus P23-45
Primary publication:
Cryo-EM structure and in vitro DNA packaging of a thermophilic virus with supersized T=7 capsids.
Bayfield OW, Klimuk E, Winkler DC, Hesketh EL, Chechik M, Cheng N, Dykeman EC, Minakhin L, Ranson NA, Severinov K, Steven AC, Antson AA
Proc Natl Acad Sci U S A 116 3556-3561 (2019)
PMID: 30737287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Portal protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 446 amino acids
Theoretical weight: 49.39 KDa
Source organism: Thermus virus P23-45
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7XXB9 (Residues: 1-438; Coverage: 98%)
Gene name: P23p86

Ligands and Environments

1 bound ligand:
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I4
Unit cell:
a: 159.081Å b: 159.081Å c: 116.913Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Global Organization and Proposed Megataxonomy of the Virus World.
Koonin et al. (2020)
4 more

3 mentions without citation

The remarkable viral portal vertex: structure and a plausible model for mechanism.
Rao et al. (2021)
2 more