6ib2

X-ray diffraction
2.1Å resolution

The structure of MKK7 in complex with the covalent 4-amino-pyrazolopyrimidine 4a

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 7 Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 36.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14733 (Residues: 101-408; Coverage: 74%)
Gene names: JNKK2, MAP2K7, MEK7, MKK7, PRKMK7, SKK4
Sequence domains: Protein kinase domain
Structure domains: Transferase(Phosphotransferase) domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 60.7Å b: 68.4Å c: 84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.25
Expression system: Escherichia coli