6ial

X-ray diffraction
1.45Å resolution

Porcine E.coli heat-labile enterotoxin B-pentamer in complex with Lacto-N-neohexaose

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Heat-labile enterotoxin B chain Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 103 amino acids
Theoretical weight: 11.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32890 (Residues: 22-124; Coverage: 100%)
Gene names: eltB, ltpB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, NAG
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 77.079Å b: 65.587Å c: 96.266Å
α: 90° β: 108.64° γ: 90°
R-values:
R R work R free
0.177 0.175 0.201
Expression system: Escherichia coli BL21(DE3)