6i94

X-ray diffraction
1.7Å resolution

R2-like ligand-binding oxidase G68L mutant with non-activated Mn/Mn cofactor (after aerobic reconstitution with Mn and Fe)

Released:

Function and Biology Details

Reaction catalysed:
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
R2-like ligand binding oxidase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 37.02 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KW80 (Residues: 1-302; Coverage: 100%)
Gene name: GK2771
Sequence domains: Ribonucleotide reductase, small chain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 55.815Å b: 97.2Å c: 126.921Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.2
Expression system: Escherichia coli BL21(DE3)