› 6i73

X-ray diffraction
1.35Å resolution

Structure of Fragaria ananassa O-methyltransferase in complex with S-adenosylhomocysteine and protocatechuic aldehyde

Released:
DOI: 10.2210/pdb6i73/pdb
PDB entry 6i73 coloured by chain, front view.
PDB entry 6i73 coloured by chain, side view.
PDB entry 6i73 coloured by chain, top view.
Source organism: Fragaria x ananassa
Entry authors: Heldner A, Schiefner A

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
caffeate O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 39.72 KDa
Source organism: Fragaria x ananassa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9M602 (Residues: 14-365; Coverage: 96%)
Gene name: omt1
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
3 bound ligands:
Ligand H6N 2 x H6N
Ligand EDO 4 x EDO
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Spacegroup: P212121
Unit cell:
a: 66.376Å b: 87.655Å c: 146.814Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.122 0.148
Expression system: Escherichia coli BL21

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