› 6i4k

X-ray diffraction
1.83Å resolution

Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Ca-ATP state

Released:
DOI: 10.2210/pdb6i4k/pdb
PDB entry 6i4k coloured by chain, front view.
PDB entry 6i4k coloured by chain, side view.
PDB entry 6i4k coloured by chain, top view.
Source organisms:
Primary publication:
Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation.
Kumpula EP, Lopez AJ, Tajedin L, Han H, Kursula I
PLoS Biol 17 e3000315 (2019)
PMID: 31199804

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146438 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin-1 Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 42.06 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8I4X0 (Residues: 1-376; Coverage: 100%)
Gene names: ACT1, ACTI, PF3D7_1246200, PFL2215w
Sequence domains: Actin
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 127 amino acids
Theoretical weight: 14.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13020 (Residues: 50-174; Coverage: 17%)
Gene names: Gsb, Gsn
Sequence domains: Gelsolin repeat

Ligands and Environments

7 bound ligands:
Ligand ATP 1 x ATP
Ligand CA 3 x CA
Ligand SCN 2 x SCN
Ligand BTB 1 x BTB
Ligand PEG 1 x PEG
Ligand CL 1 x CL
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Unit cell:
a: 69.83Å b: 71.38Å c: 110.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.197
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 review citation

Towards a structural understanding of the remodeling of the actin cytoskeleton.
Merino et al. (2020)
7 other articles

2 mentions without citation

Sulfonylpiperazine compounds prevent Plasmodium falciparum invasion of red blood cells through interference with actin-1/profilin dynamics.
Dans et al. (2023)
1 more