6i41

X-ray diffraction
1.9Å resolution

Co-crystal structure of human SPOP MATH domain (wild-type) and human BRD3 fragment

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Speckle-type POZ protein Chain: A
Molecule details ›
Chain: A
Length: 145 amino acids
Theoretical weight: 16.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43791 (Residues: 28-166; Coverage: 37%)
Gene name: SPOP
Sequence domains: MATH domain
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Bromodomain-containing protein 3 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 936 Da
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q15059 (Residues: 245-253; Coverage: 1%)
Gene names: BRD3, KIAA0043, RING3L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 61.508Å b: 55.18Å c: 42.606Å
α: 90° β: 100.39° γ: 90°
R-values:
R R work R free
0.19 0.188 0.238
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli BL21