› 6i1a

X-ray diffraction
1.27Å resolution

Crystal structure of rutinosidase from Aspergillus niger

Released:
DOI: 10.2210/pdb6i1a/pdb
PDB 6i1a coloured by chain and viewed from the front.
PDB 6i1a coloured by chain and viewed from the side.
PDB 6i1a coloured by chain and viewed from the top.
Source organism: Aspergillus niger
Primary publication:
Rutinosidase from Aspergillus niger: crystal structure and insight into the enzymatic activity.
Pachl P, Kapešová J, Brynda J, Biedermannová L, Pelantová H, Bojarová P, Křen V, Řezáčová P, Kotik M
FEBS J 287 3315-3327 (2020)
PMID: 31943739

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 370 amino acids
Theoretical weight: 41.67 KDa
Source organism: Aspergillus niger
Expression system: Komagataella pastoris
UniProt:
  • Canonical: G3YFQ1 (Residues: 18-386; Coverage: 100%)
Gene name: ASPNIDRAFT_52811
Sequence domains: Cellulase (glycosyl hydrolase family 5)

Ligands and Environments

2 bound ligands:
Ligand NAG 10 x NAG
Ligand EDO 28 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 53.068Å b: 97.359Å c: 139.259Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.178
Expression system: Komagataella pastoris

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Citations

1 review citation

Peculiarities and systematics of microbial diglycosidases, and their applications in food technology.
Baglioni et al. (2021)
3 other articles