6hyi

X-ray diffraction
1.4Å resolution

Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi at 1.4 A resolution in complex with inosine

Released:
Source organism: Trypanosoma cruzi
Entry authors: Volpato Santos Y, Lorentzen E, Basquin J, Boshart M

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase A regulatory subunit Chain: B
Molecule details ›
Chain: B
Length: 304 amino acids
Theoretical weight: 34.48 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q86RB0 (Residues: 200-503; Coverage: 60%)
Gene names: C3747_37g237, ECC02_004294, PKAR
Sequence domains: Cyclic nucleotide-binding domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 40.74Å b: 88.529Å c: 46.02Å
α: 90° β: 98.28° γ: 90°
R-values:
R R work R free
0.181 0.18 0.201
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'