› 6hyi

X-ray diffraction
1.4Å resolution

Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi at 1.4 A resolution in complex with inosine

Released:
DOI: 10.2210/pdb6hyi/pdb
PDB 6hyi coloured by chain and viewed from the front.
PDB 6hyi coloured by chain and viewed from the side.
PDB 6hyi coloured by chain and viewed from the top.
Source organism: Trypanosoma cruzi
Entry authors: Volpato Santos Y, Lorentzen E, Basquin J, Boshart M

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase A regulatory subunit Chain: B
Molecule details ›
Chain: B
Length: 304 amino acids
Theoretical weight: 34.48 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q86RB0 (Residues: 200-503; Coverage: 60%)
Gene names: C3747_37g237, ECC02_004294, PKAR
Sequence domains: Cyclic nucleotide-binding domain

Ligands and Environments

1 bound ligand:
Ligand NOS 2 x NOS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 40.74Å b: 88.529Å c: 46.02Å
α: 90° β: 98.28° γ: 90°
R-values:
R R work R free
0.181 0.18 0.201
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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