› 6hy2

X-ray diffraction
1.6Å resolution

Structure guided design of an antibacterial peptide that targets UDP-N-acetylglucosamine acyltransferase

Released:
DOI: 10.2210/pdb6hy2/pdb
PDB entry 6hy2 coloured by chain, front view.
PDB entry 6hy2 coloured by chain, side view.
PDB entry 6hy2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure guided design of an antibacterial peptide that targets UDP-N-acetylglucosamine acyltransferase.
Dangkulwanich M, Raetz CRH, Williams AH
Sci Rep 9 3947 (2019)
PMID: 30850651

Function and Biology Details

Reaction catalysed: 
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141516 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase Chain: X
Molecule details ›
Chain: X
Length: 262 amino acids
Theoretical weight: 28.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A722 (Residues: 1-262; Coverage: 100%)
Gene names: JW0176, b0181, lpxA
Sequence domains:
Structure domains:
TRP-MET-LEU-ASP-PRO-ILE-ALA-GLY-LYS-TRP-SER-ARG Chain: A
Molecule details ›
Chain: A
Length: 12 amino acids
Theoretical weight: 1.46 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU R-AXIS IV
Spacegroup: P213
Unit cell:
a: 96.809Å b: 96.809Å c: 96.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.226
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Lipid-Centric Approaches in Combating Infectious Diseases: Antibacterials, Antifungals and Antivirals with Lipid-Associated Mechanisms of Action.
Ostroumova et al. (2023)
2 more

1 mention without citation

Structure guided design of an antibacterial peptide that targets UDP-N-acetylglucosamine acyltransferase.
Dangkulwanich et al. (2019)