6hxg

X-ray diffraction
1.9Å resolution

PDX1.2/PDX1.3 complex (intermediate)

Released:

Function and Biology Details

Reaction catalysed:
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only 24-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pyridoxal 5'-phosphate synthase-like subunit PDX1.2 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 320 amino acids
Theoretical weight: 34.7 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZNR6 (Residues: 1-314; Coverage: 100%)
Gene names: A37, At3g16050, MSL1.3, PDX12, PDX1L2
Sequence domains: SOR/SNZ family
Structure domains: Aldolase class I
Pyridoxal 5'-phosphate synthase subunit PDX1.3 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 271 amino acids
Theoretical weight: 29.2 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8L940 (Residues: 21-291; Coverage: 88%)
Gene names: At5g01410, GIP2, PDX13, PDX1L3, RSR4, T10O8.120
Sequence domains: SOR/SNZ family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 177.976Å b: 177.976Å c: 116.855Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.216 0.231
Expression system: Escherichia coli BL21(DE3)