› 6hut

X-ray diffraction
1.29Å resolution

GFP8 - a stabilized variant of cycle-3 GFP

Released:
DOI: 10.2210/pdb6hut/pdb
PDB 6hut coloured by chain and viewed from the front.
PDB 6hut coloured by chain and viewed from the side.
PDB 6hut coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Entry authors: Petek M, Rangel Pereira M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.41 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 52.16Å b: 63.37Å c: 69.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.203
Expression system: Escherichia coli BL21(DE3)

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