6hup

Electron Microscopy
3.58Å resolution

CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with diazepam (Valium), GABA and megabody Mb38.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (8 distinct):
Gamma-aminobutyric acid receptor subunit alpha-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 464 amino acids
Theoretical weight: 52.92 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P14867 (Residues: 1-1, 28-456; Coverage: 94%)
  • Canonical: P08219 (Residues: 2-27; Coverage: 0%)
Gene name: GABRA1
Sequence domains:
Gamma-aminobutyric acid receptor subunit beta-3 Chains: B, E
Molecule details ›
Chains: B, E
Length: 473 amino acids
Theoretical weight: 54.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28472 (Residues: 26-473; Coverage: 100%)
  • Best match: P28472-2 (Residues: 1-473)
Gene name: GABRB3
Sequence domains:
Gamma-aminobutyric acid receptor subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 495 amino acids
Theoretical weight: 56.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P18507 (Residues: 1-376, 377-467; Coverage: 100%)
  • Best match: P18507-2 (Residues: 1-475)
Gene name: GABRG2
Sequence domains:
Megabody Mb38 Chain: G
Molecule details ›
Chain: G
Length: 539 amino acids
Theoretical weight: 57.78 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.58Å
Relevant EMDB volumes: EMD-0283
Expression systems:
  • Homo sapiens
  • Escherichia coli