6hoh

X-ray diffraction
2.25Å resolution

Structure of VPS34 LIR motif (S249E) bound to GABARAP

Released:

Function and Biology Details

Reaction catalysed:
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-aminobutyric acid receptor-associated protein; Phosphatidylinositol 3-kinase catalytic subunit type 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 129 amino acids
Theoretical weight: 15.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95166 (Residues: 2-112; Coverage: 95%)
  • Canonical: Q8NEB9 (Residues: 244-258, 262-262; Coverage: 2%)
Gene names: FLC3B, GABARAP, HT004, PIK3C3, VPS34
Sequence domains: Autophagy protein Atg8 ubiquitin like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P213
Unit cell:
a: 119.502Å b: 119.502Å c: 119.502Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.25
Expression system: Escherichia coli