› 6hmi

Solution NMR

Solution structure of the RNA duplex formed by the 5'-end of U1snRNA and the 5'-splice site of SMN2 exon7

Released:
DOI: 10.2210/pdb6hmi/pdb
PDB entry 6hmi coloured by chain, ensemble of 20 models, front view.
PDB entry 6hmi coloured by chain, ensemble of 20 models, side view.
PDB entry 6hmi coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of a small molecule targeting RNA for a specific splicing correction.
Campagne S, Boigner S, Rüdisser S, Moursy A, Gillioz L, Knörlein A, Hall J, Ratni H, Cléry A, Allain FH
Nat Chem Biol 15 1191-1198 (2019)
PMID: 31636429

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197093 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (5'-R(*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 11 nucleotides
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
RNA (5'-R(*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.55 KDa
Source organism: Homo sapiens
Expression system: synthetic construct

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PSU 2 x PSU

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 30%
Refinement method: molecular dynamics
Expression system: synthetic construct

Quick links

Citations

23 review citations

Small molecule recognition of disease-relevant RNA structures.
Meyer et al. (2020)
22 more

1 mention without citation

Principles and correction of 5'-splice site selection.
Malard et al. (2022)