6hgc

X-ray diffraction
3.02Å resolution

Structure of Calypso in complex with DEUBAD of ASX

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin carboxyl-terminal hydrolase calypso Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.47 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7K5N4 (Residues: 43-306, 334-404; Coverage: 71%)
Gene names: CG8445, calypso
Sequence domains: Ubiquitin carboxyl-terminal hydrolase, family 1
Polycomb protein Asx Chain: C
Molecule details ›
Chain: C
Length: 110 amino acids
Theoretical weight: 12.35 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9V727 (Residues: 209-318; Coverage: 7%)
Gene names: Asx, CG8787
Sequence domains: Asx homology domain
Ubiquitin carboxyl-terminal hydrolase calypso Chain: B
Molecule details ›
Chain: B
Length: 310 amino acids
Theoretical weight: 34.98 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7K5N4 (Residues: 43-306, 334-373; Coverage: 65%)
Gene names: CG8445, calypso
Sequence domains: Ubiquitin carboxyl-terminal hydrolase, family 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P6122
Unit cell:
a: 119.505Å b: 119.505Å c: 408.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.27 0.268 0.292
Expression system: Escherichia coli