› 6hee

X-ray diffraction
2.3Å resolution

Crystal structure of Extracellular Domain 1 (ECD1) of FtsX from S. pneumonie in complex with undecyl-maltoside

Released:
DOI: 10.2210/pdb6hee/pdb
PDB entry 6hee coloured by chain, front view.
PDB entry 6hee coloured by chain, side view.
PDB entry 6hee coloured by chain, top view.
Source organism: Streptococcus pneumoniae D39
Primary publication:
Structure of the Large Extracellular Loop of FtsX and Its Interaction with the Essential Peptidoglycan Hydrolase PcsB in Streptococcus pneumoniae.
Rued BE, Alcorlo M, Edmonds KA, Martínez-Caballero S, Straume D, Fu Y, Bruce KE, Wu H, Håvarstein LS, Hermoso JA, Winkler ME, Giedroc DP
mBio 10 (2019)
PMID: 30696736

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein FtsX Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Streptococcus pneumoniae D39
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04LE4 (Residues: 49-165; Coverage: 38%)
Gene names: SPD_0660, ftsX
Sequence domains: FtsX extracellular domain

Ligands and Environments

3 bound ligands:
Ligand UMQ 1 x UMQ
Ligand SO4 1 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P43212
Unit cell:
a: 84.61Å b: 84.61Å c: 106.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.263 0.261 0.298
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Uncovering the activities, biological roles, and regulation of bacterial cell wall hydrolases and tailoring enzymes.
Do et al. (2020)
5 more

1 mention without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)