› 6had

X-ray diffraction
1.04Å resolution

Human transketolase variant E160Q

Released:
DOI: 10.2210/pdb6had/pdb
PDB entry 6had coloured by chain, front view.
PDB entry 6had coloured by chain, side view.
PDB entry 6had coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Low-barrier hydrogen bonds in enzyme cooperativity.
Dai S, Funk LM, von Pappenheim FR, Sautner V, Paulikat M, Schröder B, Uranga J, Mata RA, Tittmann K
Nature 573 609-613 (2019)
PMID: 31534226

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chain: A
Molecule details ›
Chain: A
Length: 637 amino acids
Theoretical weight: 69.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29401 (Residues: 1-623; Coverage: 100%)
Gene name: TKT
Sequence domains:

Ligands and Environments


Cofactor: Ligand TPP 1 x TPP
3 bound ligands:
Ligand NA 1 x NA
Ligand CA 1 x CA
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: C2
Unit cell:
a: 115.71Å b: 85.49Å c: 73.61Å
α: 90° β: 127.85° γ: 90°
R-values:
R R work R free
0.111 0.111 0.13
Expression system: Escherichia coli

Quick links

Citations

3 review citations

On the Case of the Misplaced Hydrogens.
Kumar et al. (2021)
2 more