6h9s

X-ray diffraction
1.9Å resolution

Crystal dimeric structure of Petrotoga mobilis lactate dehydrogenase with NADH

Released:
Source organism: Petrotoga mobilis SJ95
Entry authors: Roche J, Engilberge S, Girard E, Madern D

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.38 KDa
Source organism: Petrotoga mobilis SJ95
Expression system: Escherichia coli
UniProt:
  • Canonical: A9BGZ9 (Residues: 1-307; Coverage: 100%)
Gene names: Pmob_1893, ldh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: C2
Unit cell:
a: 91.268Å b: 103.739Å c: 90.7Å
α: 90° β: 107.25° γ: 90°
R-values:
R R work R free
0.171 0.169 0.197
Expression system: Escherichia coli