› 6h9i

X-ray diffraction
2.29Å resolution

Csf5, CRISPR-Cas type IV Cas6 crRNA endonuclease

Released:
DOI: 10.2210/pdb6h9i/pdb
PDB entry 6h9i coloured by chain, front view.
PDB entry 6h9i coloured by chain, side view.
PDB entry 6h9i coloured by chain, top view.
Source organism: Aromatoleum aromaticum EbN1
Primary publication:
Type IV CRISPR RNA processing and effector complex formation in Aromatoleum aromaticum.
Özcan A, Pausch P, Linden A, Wulf A, Schühle K, Heider J, Urlaub H, Heimerl T, Bange G, Randau L
Nat Microbiol 4 89-96 (2019)
PMID: 30397343

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177738 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Csf5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 266 amino acids
Theoretical weight: 29.7 KDa
Source organism: Aromatoleum aromaticum EbN1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NWP0 (Residues: 2-259; Coverage: 100%)
Gene name: p1B372
crRNA Chains: C, E
Molecule details ›
Chains: C, E
Length: 25 nucleotides
Theoretical weight: 8.08 KDa
Source organism: Aromatoleum aromaticum EbN1
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand TLA 3 x TLA
Ligand GOL 3 x GOL
Ligand MN 1 x MN
1 modified residue:
Ligand 23G 2 x 23G

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 62.85Å b: 84.237Å c: 71.472Å
α: 90° β: 100.35° γ: 90°
R-values:
R R work R free
0.225 0.223 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants.
Makarova et al. (2020)
13 more

1 mention without citation

Structural basis of Type IV CRISPR RNA biogenesis by a Cas6 endoribonuclease.
Taylor et al. (2019)