6h7l

X-ray diffraction
2.7Å resolution

ACTIVATED TURKEY BETA1 ADRENOCEPTOR WITH BOUND PARTIAL AGONIST DOBUTAMINE AND NANOBODY Nb6B9

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thioredoxin 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 109 amino acids
Theoretical weight: 11.78 KDa
Source organism: Escherichia coli K-12
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Beta-1 adrenergic receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 35 KDa
Source organism: Meleagris gallopavo
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P07700 (Residues: 44-368; Coverage: 62%)
Gene name: ADRB1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Camelid antibody fragment Nb6B9 Chains: C, D
Molecule details ›
Chains: C, D
Length: 121 amino acids
Theoretical weight: 13.04 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 116.513Å b: 119.699Å c: 129.156Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.241 0.278
Expression systems:
  • Trichoplusia ni
  • Escherichia coli