› 6h6o

X-ray diffraction
1.7Å resolution

UbiJ-SCP2 Ubiquinone synthesis protein

Released:
DOI: 10.2210/pdb6h6o/pdb
PDB entry 6h6o coloured by chain, front view.
PDB entry 6h6o coloured by chain, side view.
PDB entry 6h6o coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone.
Hajj Chehade M, Pelosi L, Fyfe CD, Loiseau L, Rascalou B, Brugière S, Kazemzadeh K, Vo CD, Ciccone L, Aussel L, Couté Y, Fontecave M, Barras F, Lombard M, Pierrel F
Cell Chem Biol 26 482-492.e7 (2019)
PMID: 30686758

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquinone biosynthesis accessory factor UbiJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.65 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADP7 (Residues: 1-120; Coverage: 60%)
Gene names: JW3811, b3834, ubiJ, yigP
Sequence domains: SCP-2 sterol transfer family

Ligands and Environments

3 bound ligands:
Ligand LMR 2 x LMR
Ligand PEG 3 x PEG
Ligand CA 7 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 48.66Å b: 67.87Å c: 74.607Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Coenzyme Q Biosynthesis: An Update on the Origins of the Benzenoid Ring and Discovery of New Ring Precursors.
Fernández-Del-Río et al. (2021)
3 more

1 mention without citation

Towards Molecular Understanding of the Functional Role of UbiJ-UbiK2 Complex in Ubiquinone Biosynthesis by Multiscale Molecular Modelling Studies.
Launay et al. (2022)