6h5h

Solution NMR

A computationally designed dRP lyase domain reconstructed from two heterologous fragments

Released:
Source organism: synthetic construct
Entry authors: ElGamacy M, Coles M, Lupas AN

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
polb4 Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.44 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: DNA polymerase beta, N-terminal domain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 80%
Refinement method: NOESY back-calculation
Expression system: Escherichia coli BL21(DE3)