6h4i

X-ray diffraction
3.22Å resolution

Usp28 catalytic domain apo

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-188743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 28 Chains: A, C
Molecule details ›
Chains: A, C
Length: 560 amino acids
Theoretical weight: 65.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96RU2 (Residues: 148-707; Coverage: 52%)
Gene names: KIAA1515, USP28
Sequence domains: Ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: I222
Unit cell:
a: 105.538Å b: 200.518Å c: 207.244Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.196
Expression system: Escherichia coli BL21(DE3)