› 6h49

X-ray diffraction
1.8Å resolution

A polyamorous repressor: deciphering the evolutionary strategy used by the phage-inducible chromosomal islands to spread in nature.

Released:
DOI: 10.2210/pdb6h49/pdb
PDB 6h49 coloured by chain and viewed from the front.
PDB 6h49 coloured by chain and viewed from the side.
PDB 6h49 coloured by chain and viewed from the top.
Source organism: Staphylococcus aureus
Primary publication:
The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature.
Ciges-Tomas JR, Alite C, Humphrey S, Donderis J, Bowring J, Salvatella X, Penadés JR, Marina A
Nat Commun 10 3676 (2019)
PMID: 31417084

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 18.16 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F0J8 (Residues: 1-156; Coverage: 58%)
Sequence domains: Helix-turn-helix

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 132.538Å b: 34.553Å c: 36.99Å
α: 90° β: 95.96° γ: 90°
R-values:
R R work R free
0.174 0.172 0.216
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

A regulatory cascade controls Staphylococcus aureus pathogenicity island activation.
Haag et al. (2021)
3 more

2 mentions without citation

Non-canonical Staphylococcus aureus pathogenicity island repression.
Miguel-Romero et al. (2022)
1 more