› 6h3o

X-ray diffraction
2.5Å resolution

Alcohol oxidase from Phanerochaete chrysosporium mutant F101S

Released:
DOI: 10.2210/pdb6h3o/pdb
PDB entry 6h3o coloured by chain, front view.
PDB entry 6h3o coloured by chain, side view.
PDB entry 6h3o coloured by chain, top view.
Source organism: Phanerodontia chrysosporium
Primary publication:
Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.
Nguyen QT, Romero E, Dijkman WP, de Vasconcellos SP, Binda C, Mattevi A, Fraaije MW
Biochemistry 57 6209-6218 (2018)
PMID: 30272958

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 651 amino acids
Theoretical weight: 72.57 KDa
Source organism: Phanerodontia chrysosporium
Expression system: Escherichia coli
UniProt:
  • Canonical: T2M2J4 (Residues: 1-651; Coverage: 100%)
Gene name: aox1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 8 x FAD
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 112.074Å b: 203.553Å c: 116.009Å
α: 90° β: 104.64° γ: 90°
R-values:
R R work R free
0.214 0.21 0.286
Expression system: Escherichia coli

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Citations

4 review citations

Glucose Oxidase, an Enzyme "Ferrari": Its Structure, Function, Production and Properties in the Light of Various Industrial and Biotechnological Applications.
Bauer et al. (2022)
3 more

1 mention without citation

Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.
Nguyen et al. (2018)