› 6h2a

X-ray diffraction
2.54Å resolution

Structure of S70A BlaC from Mycobacterium tuberculosis obtained from crystals produced in the presence of DTT

Released:
DOI: 10.2210/pdb6h2a/pdb
PDB 6h2a coloured by chain and viewed from the front.
PDB 6h2a coloured by chain and viewed from the side.
PDB 6h2a coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis
Entry authors: Tassoni R, Pannu NS, Ubbink M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-lactamase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 274 amino acids
Theoretical weight: 29.46 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
Structure domains: DD-peptidase/beta-lactamase superfamily
Possible protein degradation product Chain: H
Molecule details ›
Chain: H
Length: 6 amino acids
Theoretical weight: 360 Da
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

4 bound ligands:
Ligand TAM 2 x TAM
Ligand DTT 4 x DTT
Ligand PO4 6 x PO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P43212
Unit cell:
a: 108.864Å b: 108.864Å c: 260.577Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.256
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography.
Pandey et al. (2021)
3 more