› 6h0d

X-ray diffraction
1.6Å resolution

Metal soaked Flv1 flavodiiron core from Synechocystis sp. PCC6803

Released:
DOI: 10.2210/pdb6h0d/pdb
PDB entry 6h0d coloured by chain, front view.
PDB entry 6h0d coloured by chain, side view.
PDB entry 6h0d coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR protein from Synechocystis sp. PCC6803.
Borges PT, Romão CV, Saraiva LM, Gonçalves VL, Carrondo MA, Teixeira M, Frazão C
J Struct Biol 205 91-102 (2019)
PMID: 30447285

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative diflavin flavoprotein A 3 Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.04 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P74373 (Residues: 26-428; Coverage: 68%)
Gene names: dfa3, sll1521
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: C2
Unit cell:
a: 76.833Å b: 86.917Å c: 72.085Å
α: 90° β: 102.07° γ: 90°
R-values:
R R work R free
0.158 0.158 0.158
Expression system: Escherichia coli

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Citations

2 review citations

ROS production and signalling in chloroplasts: cornerstones and evolving concepts.
Foyer et al. (2022)
1 more