PDBe 6gz4

Electron Microscopy
3.6Å resolution

tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-2 (TI-POST-2)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 86-mer (preferred)
Entry contents:
79 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (86 distinct):
ribosomal protein uL2 Chain: AA
Molecule details ›
Chain: AA
Length: 252 amino acids
Theoretical weight: 27.46 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS2 Chain: BA
Molecule details ›
Chain: BA
Length: 215 amino acids
Theoretical weight: 24.25 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL3 Chain: AB
Molecule details ›
Chain: AB
Length: 394 amino acids
Theoretical weight: 45.22 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS1 Chain: BB
Molecule details ›
Chain: BB
Length: 212 amino acids
Theoretical weight: 24.66 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL4 Chain: AC
Molecule details ›
Chain: AC
Length: 363 amino acids
Theoretical weight: 41.17 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS28 Chain: BC
Molecule details ›
Chain: BC
Length: 222 amino acids
Theoretical weight: 24.57 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS3 Chain: BD
Molecule details ›
Chain: BD
Length: 220 amino acids
Theoretical weight: 24.36 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS7 Chain: BF
Molecule details ›
Chain: BF
Length: 190 amino acids
Theoretical weight: 21.42 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS10 Chain: BK
Molecule details ›
Chain: BK
Length: 98 amino acids
Theoretical weight: 11.77 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS12 Chain: BM
Molecule details ›
Chain: BM
Length: 120 amino acids
Theoretical weight: 13.39 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS19 Chain: BP
Molecule details ›
Chain: BP
Length: 120 amino acids
Theoretical weight: 14.27 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS9 Chain: BQ
Molecule details ›
Chain: BQ
Length: 139 amino acids
Theoretical weight: 15.77 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS17 Chain: BR
Molecule details ›
Chain: BR
Length: 125 amino acids
Theoretical weight: 14.45 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS13 Chain: BS
Molecule details ›
Chain: BS
Length: 139 amino acids
Theoretical weight: 16.38 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS19 Chain: BT
Molecule details ›
Chain: BT
Length: 143 amino acids
Theoretical weight: 15.82 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS10 Chain: BU
Molecule details ›
Chain: BU
Length: 97 amino acids
Theoretical weight: 11.03 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS10 Chain: BZ
Molecule details ›
Chain: BZ
Length: 86 amino acids
Theoretical weight: 9.8 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS28 Chain: Bc
Molecule details ›
Chain: Bc
Length: 62 amino acids
Theoretical weight: 7.01 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS14 Chain: Bd
Molecule details ›
Chain: Bd
Length: 51 amino acids
Theoretical weight: 6.12 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS31 Chain: Bf
Molecule details ›
Chain: Bf
Length: 73 amino acids
Theoretical weight: 8.65 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein RACK 1 Chain: Bg
Molecule details ›
Chain: Bg
Length: 314 amino acids
Theoretical weight: 34.73 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS4 Chain: BE
Molecule details ›
Chain: BE
Length: 257 amino acids
Theoretical weight: 28.89 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS6 Chain: BG
Molecule details ›
Chain: BG
Length: 232 amino acids
Theoretical weight: 26.91 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS7 Chain: BH
Molecule details ›
Chain: BH
Length: 183 amino acids
Theoretical weight: 20.99 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS8 Chain: BI
Molecule details ›
Chain: BI
Length: 207 amino acids
Theoretical weight: 24.13 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS4 Chain: BJ
Molecule details ›
Chain: BJ
Length: 179 amino acids
Theoretical weight: 21.22 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS17 Chain: BL
Molecule details ›
Chain: BL
Length: 153 amino acids
Theoretical weight: 17.91 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS15 Chain: BN
Molecule details ›
Chain: BN
Length: 149 amino acids
Theoretical weight: 17.06 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS11 Chain: BO
Molecule details ›
Chain: BO
Length: 136 amino acids
Theoretical weight: 14.54 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS21 Chain: BV
Molecule details ›
Chain: BV
Length: 81 amino acids
Theoretical weight: 8.86 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS8 Chain: BW
Molecule details ›
Chain: BW
Length: 129 amino acids
Theoretical weight: 14.73 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uS12 Chain: BX
Molecule details ›
Chain: BX
Length: 141 amino acids
Theoretical weight: 15.63 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS24 Chain: BY
Molecule details ›
Chain: BY
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS26 Chain: Ba
Molecule details ›
Chain: Ba
Length: 97 amino acids
Theoretical weight: 11.09 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS27 Chain: Bb
Molecule details ›
Chain: Bb
Length: 80 amino acids
Theoretical weight: 9 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS30 Chain: Be
Molecule details ›
Chain: Be
Length: 55 amino acids
Theoretical weight: 6.24 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL18 Chain: AD
Molecule details ›
Chain: AD
Length: 294 amino acids
Theoretical weight: 34.09 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL6 Chain: AE
Molecule details ›
Chain: AE
Length: 194 amino acids
Theoretical weight: 22.26 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL30 Chain: AF
Molecule details ›
Chain: AF
Length: 234 amino acids
Theoretical weight: 27.73 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL8 Chain: AG
Molecule details ›
Chain: AG
Length: 234 amino acids
Theoretical weight: 26.72 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL6 Chain: AH
Molecule details ›
Chain: AH
Length: 191 amino acids
Theoretical weight: 21.77 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL16 Chain: AI
Molecule details ›
Chain: AI
Length: 208 amino acids
Theoretical weight: 24.15 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL5 Chain: AJ
Molecule details ›
Chain: AJ
Length: 169 amino acids
Theoretical weight: 19.27 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL13 Chain: AL
Molecule details ›
Chain: AL
Length: 205 amino acids
Theoretical weight: 23.57 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL14 Chain: AM
Molecule details ›
Chain: AM
Length: 139 amino acids
Theoretical weight: 16.22 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL15 Chain: AN
Molecule details ›
Chain: AN
Length: 203 amino acids
Theoretical weight: 24.08 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL13 Chain: AO
Molecule details ›
Chain: AO
Length: 195 amino acids
Theoretical weight: 22.82 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL22 Chain: AP
Molecule details ›
Chain: AP
Length: 153 amino acids
Theoretical weight: 17.76 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL18 Chain: AQ
Molecule details ›
Chain: AQ
Length: 187 amino acids
Theoretical weight: 21.56 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL19 Chain: AR
Molecule details ›
Chain: AR
Length: 181 amino acids
Theoretical weight: 21.74 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL20 Chain: AS
Molecule details ›
Chain: AS
Length: 175 amino acids
Theoretical weight: 20.66 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL21 Chain: AT
Molecule details ›
Chain: AT
Length: 157 amino acids
Theoretical weight: 18.28 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL22 Chain: AU
Molecule details ›
Chain: AU
Length: 99 amino acids
Theoretical weight: 11.48 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL14 Chain: AV
Molecule details ›
Chain: AV
Length: 129 amino acids
Theoretical weight: 13.83 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL24 Chain: AW
Molecule details ›
Chain: AW
Length: 121 amino acids
Theoretical weight: 14.09 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL23 Chain: AX
Molecule details ›
Chain: AX
Length: 117 amino acids
Theoretical weight: 13.61 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL24 Chain: AY
Molecule details ›
Chain: AY
Length: 127 amino acids
Theoretical weight: 15.16 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL27 Chain: AZ
Molecule details ›
Chain: AZ
Length: 134 amino acids
Theoretical weight: 15.65 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL15 Chain: Aa
Molecule details ›
Chain: Aa
Length: 147 amino acids
Theoretical weight: 16.47 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL29 Chain: Ab
Molecule details ›
Chain: Ab
Length: 68 amino acids
Theoretical weight: 8 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL30 Chain: Ac
Molecule details ›
Chain: Ac
Length: 103 amino acids
Theoretical weight: 11.5 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL31 Chain: Ad
Molecule details ›
Chain: Ad
Length: 106 amino acids
Theoretical weight: 12.51 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL32 Chain: Ae
Molecule details ›
Chain: Ae
Length: 129 amino acids
Theoretical weight: 15.18 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL33 Chain: Af
Molecule details ›
Chain: Af
Length: 109 amino acids
Theoretical weight: 12.43 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL34 Chain: Ag
Molecule details ›
Chain: Ag
Length: 114 amino acids
Theoretical weight: 12.99 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL29 Chain: Ah
Molecule details ›
Chain: Ah
Length: 122 amino acids
Theoretical weight: 14.46 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL36 Chain: Ai
Molecule details ›
Chain: Ai
Length: 97 amino acids
Theoretical weight: 11.37 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL37 Chain: Aj
Molecule details ›
Chain: Aj
Length: 84 amino acids
Theoretical weight: 9.86 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL38 Chain: Ak
Molecule details ›
Chain: Ak
Length: 69 amino acids
Theoretical weight: 8.11 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL39 Chain: Al
Molecule details ›
Chain: Al
Length: 50 amino acids
Theoretical weight: 6.3 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL40 Chain: Am
Molecule details ›
Chain: Am
Length: 50 amino acids
Theoretical weight: 5.96 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL41 Chain: An
Molecule details ›
Chain: An
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL42 Chain: Ao
Molecule details ›
Chain: Ao
Length: 105 amino acids
Theoretical weight: 12.35 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL43 Chain: Ap
Molecule details ›
Chain: Ap
Length: 91 amino acids
Theoretical weight: 10.17 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL28 Chain: At
Molecule details ›
Chain: At
Length: 122 amino acids
Theoretical weight: 13.99 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL1 Chain: Au
Molecule details ›
Chain: Au
Length: 217 amino acids
Theoretical weight: 24.88 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL11 Chain: Aq
Molecule details ›
Chain: Aq
Length: 151 amino acids
Theoretical weight: 16.29 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein uL10 Chain: AK
Molecule details ›
Chain: AK
Length: 202 amino acids
Theoretical weight: 22.22 KDa
Source organism: Oryctolagus cuniculus
eukaryotic elongation factor 2 (eEF2) Chain: Ct
Molecule details ›
Chain: Ct
Length: 853 amino acids
Theoretical weight: 95.06 KDa
Source organism: Oryctolagus cuniculus
28S ribosomal RNA Chain: A2
Molecule details ›
Chain: A2
Length: 3612 nucleotides
Theoretical weight: 1170.16 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
ap/P-site tRNA Chain: Bv
Molecule details ›
Chain: Bv
Length: 76 nucleotides
Theoretical weight: 24.44 KDa
Sequence domains: tRNA
Messenger RNA Chain: Bx
Molecule details ›
Chain: Bx
Length: 11 nucleotides
Theoretical weight: 3.49 KDa
pe/E-site-tRNA Chain: Bw
Molecule details ›
Chain: Bw
Length: 76 nucleotides
Theoretical weight: 24.53 KDa
Sequence domains: tRNA
18S Ribosomal RNA Chain: B1
Molecule details ›
Chain: B1
Length: 1708 nucleotides
Theoretical weight: 551.11 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
5.8S ribosomal RNA Chain: A3
Molecule details ›
Chain: A3
Length: 157 nucleotides
Theoretical weight: 50.45 KDa
Sequence domains: 5.8S ribosomal RNA
5S ribosomal RNA Chain: A4
Molecule details ›
Chain: A4
Length: 119 nucleotides
Theoretical weight: 38.39 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-0099