Structure analysis

Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State III)

Electron Microscopy
3.9Å resolution
Source organisms:
Assembly composition:
hetero 58-mer (preferred)
Entry contents: 53 distinct polypeptide molecules
5 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 58-mer
Accessible surface area: 841375.53 Å2
Buried surface area: 271741.97 Å2
Dissociation area: 3,368.94 Å2
Dissociation energy (ΔGdiss): -7.62 kcal/mol
Dissociation entropy (TΔSdiss): 31.56 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135504

Macromolecules

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Chain: E
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Gene names: JW3281, b3319, eryA, rplD
Pfam: Ribosomal protein L4/L1 family
InterPro:

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Chain: J
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AA10 (Residues: 1-142; Coverage: 100%)
Gene names: JW3200, b3231, rplM
Pfam: Ribosomal protein L13
InterPro:

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Chain: N
Length: 120 amino acids
Theoretical weight: 13.72 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG44 (Residues: 1-120; Coverage: 95%)
Gene names: JW3256, b3294, rplQ
Pfam: Ribosomal protein L17
InterPro:

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Chain: O
Length: 116 amino acids
Theoretical weight: 12.66 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0C018 (Residues: 2-117; Coverage: 99%)
Gene names: JW3266, b3304, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
InterPro:

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Chain: P
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7K6 (Residues: 2-115; Coverage: 99%)
Gene names: JW2587, b2606, rplS
Pfam: Ribosomal protein L19
InterPro:

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Chain: Q
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L3 (Residues: 2-118; Coverage: 99%)
Gene names: JW1706, b1716, pdzA, rplT
Pfam: Ribosomal protein L20
InterPro:

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Chain: R
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG48 (Residues: 1-103; Coverage: 100%)
Gene names: JW3153, b3186, rplU
Pfam: Ribosomal prokaryotic L21 protein
InterPro:

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Chain: T
Length: 93 amino acids
Theoretical weight: 10.55 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 1-93; Coverage: 93%)
Gene names: JW3280, b3318, rplW
Pfam: Ribosomal protein L23
InterPro:

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Chain: W
Length: 75 amino acids
Theoretical weight: 8.17 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 11-85; Coverage: 88%)
Gene names: JW3152, b3185, rpmA
Pfam: Ribosomal L27 protein
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Chain: Y
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Gene names: JW3274, b3312, rpmC
Pfam: Ribosomal L29 protein
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Chain: 0
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N4 (Residues: 2-57; Coverage: 98%)
Gene names: JW1075, b1089, rpmF
Pfam: Ribosomal L32p protein family
InterPro:

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Chain: 2
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Pfam: Ribosomal protein L34
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Chain: 4
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q6 (Residues: 1-38; Coverage: 100%)
Gene names: JW3261, b3299, rpmJ
Pfam: Ribosomal protein L36
InterPro:

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Chain: h
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 2-130; Coverage: 99%)
Gene names: JW3268, b3306, rpsH
Pfam: Ribosomal protein S8
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Chain: k
Length: 116 amino acids
Theoretical weight: 12.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 13-128; Coverage: 90%)
Gene names: JW3259, b3297, rpsK
Pfam: Ribosomal protein S11
InterPro:

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Chain: l
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: n
Length: 101 amino acids
Theoretical weight: 11.55 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 2-35, 36-101; Coverage: 99%)
Gene names: JW3269, b3307, rpsN
Pfam: Ribosomal protein S14p/S29e
InterPro:

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Chain: o
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 2-89; Coverage: 99%)
Gene names: JW3134, b3165, rpsO, secC
Pfam: Ribosomal protein S15
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Chain: p
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Pfam: Ribosomal protein S16
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Chain: q
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 4-83; Coverage: 95%)
Gene names: JW3273, b3311, neaA, rpsQ
Pfam: Ribosomal protein S17
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Chain: r
Length: 65 amino acids
Theoretical weight: 7.61 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 10-74; Coverage: 87%)
Gene names: JW4160, b4202, rpsR
Pfam: Ribosomal protein S18
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Chain: t
Length: 85 amino acids
Theoretical weight: 9.51 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 3-87; Coverage: 98%)
Gene names: JW0022, b0023, rpsT
Pfam: Ribosomal protein S20
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Chain: u
Length: 65 amino acids
Theoretical weight: 7.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 4-68; Coverage: 92%)
Gene names: JW3037, b3065, rpsU
Pfam: Ribosomal protein S21
InterPro:

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Chain: v
Length: 350 amino acids
Theoretical weight: 39.49 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7I0 (Residues: 2-351; Coverage: 97%)
Gene names: JW1202, b1211, prfA, sueB, uar
Pfam:
InterPro:

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Chain: z
Length: 14 amino acids
Theoretical weight: 1.7 KDa
Source organism: Apis mellifera
Expression system: Not provided
UniProt:
  • Canonical: Q8WSY8 (Residues: 158-171; Coverage: 7%)
Pfam: Apidaecin
InterPro: Apidaecin

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Name: 23S ribosomal RNA
Representative chains: A
Length: 2903 nucleotides
Theoretical weight: 941.32 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: B
Length: 120 nucleotides
Theoretical weight: 38.81 KDa
Rfam: 5S ribosomal RNA

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Name: Messenger RNA
Representative chains: 7
Length: 7 nucleotides
Theoretical weight: 2.23 KDa

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Name: 16S ribosomal RNA
Representative chains: a
Length: 1539 nucleotides
Theoretical weight: 498.73 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: fMet-tRNA
Representative chains: x
Length: 77 nucleotides
Theoretical weight: 24.8 KDa
Rfam: tRNA

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