› 6gx6

X-ray diffraction
2Å resolution

Crystal structure of IMP3 RRM12 in complex with RNA (ACAC)

Released:
DOI: 10.2210/pdb6gx6/pdb
PDB entry 6gx6 coloured by chain, front view.
PDB entry 6gx6 coloured by chain, side view.
PDB entry 6gx6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of IMP3 RRM12 recognition of RNA.
Jia M, Gut H, Chao JA
RNA 24 1659-1666 (2018)
PMID: 30135093

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126274 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Insulin-like growth factor 2 mRNA-binding protein 3 Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O00425 (Residues: 1-161; Coverage: 28%)
Gene names: IGF2BP3, IMP3, KOC1, VICKZ3
Sequence domains: RNA recognition motif
RNA (5'-R(*AP*CP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.22 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 75.44Å b: 75.44Å c: 66.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.236
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

IGF2BP3 From Physiology to Cancer: Novel Discoveries, Unsolved Issues, and Future Perspectives.
Mancarella et al. (2019)
3 more

3 mentions without citation

Detecting protein and DNA/RNA structures in cryo-EM maps of intermediate resolution using deep learning.
Wang et al. (2021)
2 more