X-ray diffraction
1.77Å resolution

Alpha-galactosidase from Thermotoga maritima in complex with cyclohexene-based carbasugar mimic of galactose covalently linked to the nucleophile


Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Alpha-galactosidase Chain: A
Molecule details ›
Chain: A
Length: 575 amino acids
Theoretical weight: 66.2 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: G4FEF4 (Residues: 1-552; Coverage: 100%)
Gene names: TM_1192, Tmari_1199, galA
Sequence domains: Melibiase
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 66.73Å b: 95.87Å c: 97.44Å
α: 90° β: 90° γ: 90°
R R work R free
0.17 0.167 0.215
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'