› 6gv2

X-ray diffraction
2.1Å resolution

Sulfolobus solfataricus 2-keto-3-deoxygluconate aldolase Y103F,Y130F,A198F variant in complex with L-2-keto, 3-deoxy-galactonate

Released:
DOI: 10.2210/pdb6gv2/pdb
PDB entry 6gv2 coloured by chain, front view.
PDB entry 6gv2 coloured by chain, side view.
PDB entry 6gv2 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Entry authors: Crennell SJ, Danson MJ, Royer S

Function and Biology Details

Reaction catalysed: 
2-dehydro-3-deoxy-6-phospho-D-galactonate = pyruvate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 33.2 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O54288 (Residues: 1-294; Coverage: 100%)
Sequence domains: Dihydrodipicolinate synthetase family

Ligands and Environments

4 bound ligands:
Ligand SSH 2 x SSH
Ligand GXV 2 x GXV
Ligand EDO 2 x EDO
Ligand GOL 7 x GOL
1 modified residue:
Ligand KPI 2 x KPI

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6522
Unit cell:
a: 103.478Å b: 103.478Å c: 245.551Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.164 0.212
Expression system: Escherichia coli BL21(DE3)

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