6gum

X-ray diffraction
1.79Å resolution

Structure of the A.thaliana E1 UFD domain in complex with E2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SUMO-conjugating enzyme SCE1 Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.18 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q42551 (Residues: 1-160; Coverage: 100%)
Gene names: AHUS5, At3g57870, EMB1637, SCE1, T10K17.80
Sequence domains: Ubiquitin-conjugating enzyme
SUMO-activating enzyme subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 209 amino acids
Theoretical weight: 23.34 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SJT1 (Residues: 437-700; Coverage: 27%)
  • Best match: Q9SJT1-2 (Residues: 437-625)
Gene names: At2g21470, EMB2764, F3K23.23, SAE2
Sequence domains: Ubiquitin/SUMO-activating enzyme ubiquitin-like domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 36.158Å b: 58.817Å c: 70.23Å
α: 90° β: 92.05° γ: 90°
R-values:
R R work R free
0.18 0.178 0.22
Expression system: Escherichia coli