6gu0

X-ray diffraction
2.5Å resolution

Crystal structure of a FimH*DsG complex from E.coli F18 with bound dimannoside Man(alpha1-3)Man in space group P213

Released:
Entry authors: Jakob RP, Sauer MM, Luber T, Canonica F, Navarra G, Ernst B, Unverzagt C, Maier T, Glockshuber R

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
FimH protein Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 29.05 KDa
Source organism: Escherichia coli F18+
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0R4I961 (Residues: 1-279; Coverage: 100%)
Gene names: ECP_4655, fimh
Sequence domains:
Structure domains: Fimbrial-type adhesion domain
FimG protein Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.42 KDa
Source organism: Escherichia coli 536
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: MMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P213
Unit cell:
a: 128.63Å b: 128.63Å c: 128.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.192
Expression systems:
  • Escherichia coli
  • Not provided