› 6gts

X-ray diffraction
3.36Å resolution

Structure of the AtaT-AtaR complex bound DNA

Released:
DOI: 10.2210/pdb6gts/pdb
PDB entry 6gts coloured by chain, front view.
PDB entry 6gts coloured by chain, side view.
PDB entry 6gts coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanism of regulation and neutralization of the AtaR-AtaT toxin-antitoxin system.
Jurėnas D, Van Melderen L, Garcia-Pino A
Nat Chem Biol 15 285-294 (2019)
PMID: 30718814

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102325 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
N-acetyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0K4I8K2 (Residues: 1-175; Coverage: 100%)
Gene names: FORC44_0349, FPS11_03015, HLZ50_06215
Sequence domains: Acetyltransferase (GNAT) domain
Toxin-antitoxin system, antitoxin component Chains: B, C
Molecule details ›
Chains: B, C
Length: 88 amino acids
Theoretical weight: 9.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: J7QA90 (Residues: 1-88; Coverage: 100%)
Gene names: ACN81_27750, BK292_07330, CF22_001922, DS732_25100, E5H86_05315, FJQ40_03795, FORC44_0348, FPS11_03010, GRW56_23065, GRW57_20865, GUC01_13975, HHH44_000669, HLX92_24800, NCTC10418_00491, NCTC10865_00468
Sequence domains: Protein of unknown function (DUF1778)
DNA Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.75 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 75.729Å b: 87.92Å c: 190.481Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.264 0.289
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

6 review citations

Small-Molecule Acetylation by GCN5-Related N-Acetyltransferases in Bacteria.
Burckhardt et al. (2020)
5 more