› 6gt5

X-ray diffraction
2.45Å resolution

METTL16 MTase domain (crystal form 2)

Released:
DOI: 10.2210/pdb6gt5/pdb
PDB 6gt5 coloured by chain and viewed from the front.
PDB 6gt5 coloured by chain and viewed from the side.
PDB 6gt5 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Methylation of Structured RNA by the m6A Writer METTL16 Is Essential for Mouse Embryonic Development.
Mendel M, Chen KM, Homolka D, Gos P, Pandey RR, McCarthy AA, Pillai RS
Mol. Cell (2018)
PMID: 30197299

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
S-adenosyl-L-methionine + adenine in U6 snRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in U6 snRNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA N6-adenosine-methyltransferase METTL16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 33.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86W50 (Residues: 1-291; Coverage: 52%)
Gene names: METT10D, METTL16
Sequence domains: RNA methyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P41212
Unit cell:
a: 89.55Å b: 89.55Å c: 179.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

46 review citations

METTLing in Stem Cell and Cancer Biology.
Tooley et al. (2023)
45 more

1 mention without citation

A comprehensive review of m6A/m6Am RNA methyltransferase structures.
Oerum et al. (2021)