› 6gs7

X-ray diffraction
3.3Å resolution

Crystal structure of peptide transporter DtpA-nanobody in glycine buffer

Released:
DOI: 10.2210/pdb6gs7/pdb
PDB entry 6gs7 coloured by chain, front view.
PDB entry 6gs7 coloured by chain, side view.
PDB entry 6gs7 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1.
Ural-Blimke Y, Flayhan A, Strauss J, Rantos V, Bartels K, Nielsen R, Pardon E, Steyaert J, Kosinski J, Quistgaard EM, Löw C
J Am Chem Soc 141 2404-2412 (2019)
PMID: 30644743

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211513 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dipeptide and tripeptide permease A Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 54.97 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77304 (Residues: 2-500; Coverage: 100%)
Gene names: JW1626, b1634, dtpA, tppB, ydgR
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains
nanobody Chain: H
Molecule details ›
Chain: H
Length: 132 amino acids
Theoretical weight: 14.4 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P212121
Unit cell:
a: 55.145Å b: 120.534Å c: 163.433Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.246
Expression system: Escherichia coli

Quick links

Citations

3 review citations

New Horizons in Structural Biology of Membrane Proteins: Experimental Evaluation of the Role of Conformational Dynamics and Intrinsic Flexibility.
Puthenveetil et al. (2022)
2 more

2 mentions without citation

Recent advances in understanding prodrug transport through the SLC15 family of proton-coupled transporters.
Minhas et al. (2020)
1 more