› 6grm

X-ray diffraction
2.3Å resolution

Structure of GFPmut2 crystallized at pH 6 and transferred to pH 9

Released:
DOI: 10.2210/pdb6grm/pdb
PDB 6grm coloured by chain and viewed from the front.
PDB 6grm coloured by chain and viewed from the side.
PDB 6grm coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Insight into GFPmut2 pH Dependence by Single Crystal Microspectrophotometry and X-ray Crystallography.
Lolli G, Raboni S, Pasqualetto E, Benoni R, Campanini B, Ronda L, Mozzarelli A, Bettati S, Battistutta R
J Phys Chem B 122 11326-11337 (2018)
PMID: 30179482

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.43 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 31.323Å b: 59.686Å c: 108.407Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.275
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Mitochondrial Reactive Oxygen Species Generated at the Complex-II Matrix or Intermembrane Space Microdomain Have Distinct Effects on Redox Signaling and Stress Sensitivity in Caenorhabditis elegans.
Trewin et al. (2019)