› 6gq3

X-ray diffraction
1.85Å resolution

Human asparagine synthetase (ASNS) in complex with 6-diazo-5-oxo-L-norleucine (DON) at 1.85 A resolution

Released:
DOI: 10.2210/pdb6gq3/pdb
PDB entry 6gq3 coloured by chain, front view.
PDB entry 6gq3 coloured by chain, side view.
PDB entry 6gq3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution crystal structure of human asparagine synthetase enables analysis of inhibitor binding and selectivity.
Zhu W, Radadiya A, Bisson C, Wenzel S, Nordin BE, Martínez-Márquez F, Imasaki T, Sedelnikova SE, Coricello A, Baumann P, Berry AH, Nomanbhoy TK, Kozarich JW, Jin Y, Rice DW, Takagi Y, Richards NGJ
Commun Biol 2 345 (2019)
PMID: 31552298

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140060 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Asparagine synthetase [glutamine-hydrolyzing] Chains: A, B
Molecule details ›
Chains: A, B
Length: 561 amino acids
Theoretical weight: 64.46 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08243 (Residues: 1-561; Coverage: 100%)
Gene names: ASNS, TS11
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ONL 2 x ONL
Ligand EDO 4 x EDO
Ligand EPE 2 x EPE
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 64.7Å b: 83.52Å c: 110.29Å
α: 90° β: 90.65° γ: 90°
R-values:
R R work R free
0.18 0.178 0.225
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Asparagine Synthetase in Cancer: Beyond Acute Lymphoblastic Leukemia.
Chiu et al. (2019)
1 more

10 mentions without citation

The Human Ntn-Hydrolase Superfamily: Structure, Functions and Perspectives.
Linhorst et al. (2022)
9 more