6gm3

X-ray diffraction
2.22Å resolution

[FeFe]-hydrogenase CpI from Clostridium pasteurianum, variant R286A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron hydrogenase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 584 amino acids
Theoretical weight: 65.03 KDa
Source organism: Clostridium pasteurianum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29166 (Residues: 1-574; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 89.81Å b: 72.89Å c: 103.14Å
α: 90° β: 96.11° γ: 90°
R-values:
R R work R free
0.187 0.184 0.23
Expression system: Escherichia coli BL21(DE3)