6gjh

X-ray diffraction
2.1Å resolution

Human Hsp27 (HspB1) alpha-crystallin domain in complex with a peptide mimic of its phosphorylatable N-terminal region

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dodecamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Heat shock protein beta-1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 87 amino acids
Theoretical weight: 9.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04792 (Residues: 84-170; Coverage: 42%)
Gene names: HSP27, HSP28, HSPB1
ALA-LEU-SER-ARG-GLN Chain: K
Molecule details ›
Chain: K
Length: 5 amino acids
Theoretical weight: 575 Da
Source organism: Homo sapiens
Expression system: Not provided
LEU-SER-GLY-VAL Chain: J
Molecule details ›
Chain: J
Length: 4 amino acids
Theoretical weight: 374 Da
Source organism: Homo sapiens
Expression system: Not provided
ALA-LEU-SER-ARG Chains: I, L
Molecule details ›
Chains: I, L
Length: 4 amino acids
Theoretical weight: 447 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 56.81Å b: 157.67Å c: 57.87Å
α: 90° β: 95.45° γ: 90°
R-values:
R R work R free
0.213 0.211 0.251
Expression systems:
  • Escherichia coli
  • Not provided