› 6gja

X-ray diffraction
1.5Å resolution

PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 - H229A MUTANT

Released:
DOI: 10.2210/pdb6gja/pdb
PDB entry 6gja coloured by chain, front view.
PDB entry 6gja coloured by chain, side view.
PDB entry 6gja coloured by chain, top view.
Source organism: Triticum aestivum
Primary publication:
Structure of a cereal purple acid phytase provides new insights to phytate degradation in plants.
Faba-Rodriguez R, Gu Y, Salmon M, Dionisio G, Brinch-Pedersen H, Brearley CA, Hemmings AM
OpenAccess logo Plant Commun 3 100305 (2022)
PMID: 35529950

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Purple acid phosphatase Chain: A
Molecule details ›
Chain: A
Length: 516 amino acids
Theoretical weight: 57.48 KDa
Source organism: Triticum aestivum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: F6MIW5 (Residues: 21-530; Coverage: 99%)
Gene names: CFC21_040935, CFC21_040939, TRAES_3BF026700100CFD_c1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
7 bound ligands:
Ligand FE 2 x FE
Ligand NAG 6 x NAG
Ligand PO4 3 x PO4
Ligand PEG 5 x PEG
Ligand EDO 8 x EDO
Ligand PGE 3 x PGE
Ligand PG6 1 x PG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R3
Unit cell:
a: 125.98Å b: 125.98Å c: 106.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.128 0.152
Expression system: Komagataella pastoris

Quick links

Citations

2 citation in other articles

Insights to the Structural Basis for the Stereospecificity of the Escherichia coli Phytase, AppA.
Acquistapace et al. (2022)
1 more